@article{111151,
  keywords = {membranes, Gram-Negative Bacteria, Lipid Bilayers, Bacterial Outer Membrane Proteins, Lipopolysaccharides, Organelle Biogenesis},
  author = {Anna Konovalova and Daniel Kahne and Thomas Silhavy},
  title = {Outer Membrane Biogenesis},
  abstract = {<p>The hallmark of gram-negative bacteria and organelles such as mitochondria and chloroplasts is the presence of an outer membrane. In bacteria such as Escherichia coli, the outer membrane is a unique asymmetric lipid bilayer with lipopolysaccharide in the outer leaflet. Integral transmembrane proteins assume a β-barrel structure, and their assembly is catalyzed by the heteropentameric Bam complex containing the outer membrane protein BamA and four lipoproteins, BamB-E. How the Bam complex assembles a great diversity of outer membrane proteins into a membrane without an obvious energy source is a particularly challenging problem, because folding intermediates are predicted to be unstable in either an aqueous or a hydrophobic environment. Two models have been put forward: the budding model, based largely on structural data, and the BamA assisted model, based on genetic and biochemical studies. Here we offer a critical discussion of the pros and cons of each.</p>
},
  year = {2017},
  journal = {Annu Rev Microbiol},
  volume = {71},
  pages = {539-556},
  month = {09/2017},
  issn = {1545-3251},
  doi = {10.1146/annurev-micro-090816-093754},
  language = {eng},
}