@article{199166,
  keywords = {Escherichia coli, Phenotype, DNA Transposable Elements, Transduction, Genetic, Lac Operon, Bacteriophage lambda, Kanamycin, R Factors},
  author = {Bremer and Silhavy and Weinstock},
  title = {Transposable lambda placMu bacteriophages for creating lacZ operon fusions and kanamycin resistance insertions in Escherichia coli},
  abstract = {<p>We have constructed several derivatives of bacteriophage lambda that translocate by using the transposition machinery of phage Mu (lambda placMu phages). Each phage carries the c end of Mu, containing the Mu cIts62, ner (cII), and A genes, and the terminal sequences from the Mu S end (beta end). These sequences contain the Mu attachment sites, and their orientation allows the lambda genome to be inserted into other chromosomes, resulting in a lambda prophage flanked by the Mu c and S sequences. These phages provide a means to isolate cells containing fusions of the lac operon to other genes in vivo in a single step. In lambda placMu50, the lacZ and lacY genes, lacking a promoter, were located adjacent to the Mu S sequence. Insertion of lambda placMu50 into a gene in the proper orientation created an operon fusion in which lacZ and lacY were expressed from the promoter of the target gene. We also introduced a gene, kan, which confers kanamycin resistance, into lambda placMu50 and lambda placMu1, an analogous phage for constructing lacZ protein fusions (Bremer et al., J. Bacteriol. 158:1084-1093, 1984). The kan gene, located between the cIII and ssb genes of lambda, permitted cells containing insertions of these phages to be selected independently of their Lac phenotype.</p>
},
  year = {1985},
  journal = {J Bacteriol},
  volume = {162},
  pages = {1092-9},
  month = {06/1985},
  issn = {0021-9193},
  doi = {10.1128/jb.162.3.1092-1099.1985},
  language = {eng},
}