@article{199291, keywords = {Bacterial Proteins, Gene Expression Regulation, Escherichia coli, Membrane Proteins, Cloning, Molecular, Genes, Regulator, Porins}, author = {Taylor and Hall and Enquist and Silhavy}, title = {Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12}, abstract = {

We report here on the cloning of a gene located within the ompB locus of E. coli K-12. This gene, designated ompR, resides on a 10.9-kilobase EcoRI fragment that we cloned, using a lambda vector and in vitro packaging techniques. By subcloning portions of this fragment into the high-copy-number plasmid pBR322, we have isolated the ompR gene on a 1.4-kilobase EcoRI-AvaI fragment. This fragment has been characterized physically and will facilitate a more detailed study of the role and mechanism of porin regulation by the ompB locus.

}, year = {1981}, journal = {J Bacteriol}, volume = {147}, pages = {255-8}, month = {07/1981}, issn = {0021-9193}, doi = {10.1128/jb.147.1.255-258.1981}, language = {eng}, }