@article{199421,
  keywords = {Kinetics, Spectrophotometry, Ultraviolet, Escherichia coli, Protein Binding, Ligands, Carrier Proteins, Glucose, Half-Life, Spectrometry, Fluorescence, Tryptophan, Galactose, Dialysis, Sepharose},
  author = {Silhavy and Boos},
  title = {The "hidden ligand" of the galactose-binding protein},
  abstract = {<p>Following tryptophan fluorescence of the galactose-binding during dissociation of the ligand it has been found that glucose dissociates with a half life of less than 5 s. Similarly, fast dissociation was also observed by following release of radioactively labelled glucose from Sepharose-coupled galactose-binding protein upon dilution. Accordingly, a previous claim that the galactose-binding protein contains glucose as a non-dissociable "hidden ligand" [G. Richarme and A. Kepes (1974) Eur. J. Biochem. 45, 127-133] has to be reinterpreted</p>
},
  year = {1975},
  journal = {Eur J Biochem},
  volume = {54},
  pages = {163-7},
  month = {05/1975},
  issn = {0014-2956},
  doi = {10.1111/j.1432-1033.1975.tb04125.x},
  language = {eng},
}