@article{199431,
  keywords = {Kinetics, Mutation, Escherichia coli, Biological Transport, Active, Transduction, Genetic, Mutagens, Galactose, Galactosidases, Glycerolphosphate Dehydrogenase, Glycerol, Methylgalactosides, Autoradiography, Carbohydrate Epimerases, Carbon Radioisotopes, Chromatography, Paper, Glycosides, Methylglycosides, Nitrosoguanidines, Succinates},
  author = {Silhavy and Boos},
  title = {Selection procedure for mutants defective in the beta-methylgalactoside transport system of Escherichia coli utilizing the compound 2R-glyceryl-beta-D-galactopyranoside},
  abstract = {<p>A procedure has been devised that allows selection of mutants defective in the beta-methylgalactoside transport system (mgl) of Escherichia coli. This procedure utilizes the compound 2R-glyceryl-beta-d-galactopyranoside (glycerylgalactoside), which is known to be transported by only two transport system in E. coli, namely, the lactose and the beta-methylgalactoside transport systems. Mutants lacking glycerol-3-phosphate dehydrogenase (glpD) are sensitive to glycerol. Similarly, mutants lacking uridine diphosphate-galactose-4-epimerase (galE) are sensitive to galactose. Glycerylgalactoside is an inducer of the lactose operon and also a substrate for beta-galactosidase. Thus, a mgl(+)glpD galE lacY strain will not grow in the presence of glycerylgalactoside owing to accumulated glycerol-3-phosphate, galactose-1-phosphate, and uridine diphosphate-galactose. We have constructed such a strain and shown that mgl mutants can be obtained by selecting for those that grow in the presence of glycerylgalactoside.</p>
},
  year = {1974},
  journal = {J Bacteriol},
  volume = {120},
  pages = {424-32},
  month = {10/1974},
  issn = {0021-9193},
  doi = {10.1128/jb.120.1.424-432.1974},
  language = {eng},
}