@article{89846,
  keywords = {Base Sequence, Mutation, Escherichia coli, Genes, Bacterial, DNA, Bacterial, Amino Acid Sequence, Escherichia coli Proteins, Cell Membrane, Macromolecular Substances, Molecular Weight, Bacterial Outer Membrane Proteins, Membrane Lipids},
  author = {Martin Braun and Thomas Silhavy},
  title = {Imp/OstA is required for cell envelope biogenesis in Escherichia coli},
  abstract = {<p>In Gram-negative bacteria, all components of the outer membrane are synthesized in the cytoplasm or the cytoplasmic leaflet of the inner membrane and must thus traverse the inner membrane and the periplasm on the way to their final destination. In this study, we show Imp/OstA to have characteristics typical for proteins involved in envelope biogenesis. Imp is essential and forms a high-molecular-weight disulphide-bonded complex in the outer membrane. Upon depletion of Imp, lipids and outer membrane proteins appear in a novel membrane fraction with higher density than the outer membrane. We propose Imp to be part of a targeting/usher system for components of the outer membrane.</p>
},
  year = {2002},
  journal = {Mol Microbiol},
  volume = {45},
  pages = {1289-302},
  month = {09/2002},
  issn = {0950-382X},
  doi = {10.1046/j.1365-2958.2002.03091.x},
  language = {eng},
}