@article{90051,
  keywords = {Base Sequence, Escherichia coli, Genes, Bacterial, Molecular Sequence Data, Transcription, Genetic, Gene Expression Regulation, Bacterial, Recombinant Fusion Proteins, Mutagenesis, Site-Directed, Macromolecular Substances, beta-Galactosidase, DNA-Directed RNA Polymerases, Genotype, Alleles, Polymerase Chain Reaction, Bacterial Outer Membrane Proteins, Oligodeoxyribonucleotides, Porins},
  author = {Slauch and Russo and Silhavy},
  title = {Suppressor mutations in rpoA suggest that OmpR controls transcription by direct interaction with the alpha subunit of RNA polymerase},
  abstract = {<p>We have isolated mutations in rpoA, the gene encoding the alpha subunit of RNA polymerase, that specifically affect transcriptional control by OmpR and EnvZ, the two-component regulatory system that controls porin gene expression in Escherichia coli. Characterization of these mutations and a previously isolated rpoA allele suggests that both positive and negative regulation of porin gene transcription involves a direct interaction between OmpR and RNA polymerase through the alpha subunit. Several of the rpoA mutations cluster in the carboxy-terminal portion of the alpha protein, further suggesting that it is this domain of alpha that is involved in interaction with OmpR and perhaps other transcriptional regulators as well.</p>
},
  year = {1991},
  journal = {J Bacteriol},
  volume = {173},
  pages = {7501-10},
  month = {12/1991},
  issn = {0021-9193},
  doi = {10.1128/jb.173.23.7501-7510.1991},
  language = {eng},
}